Biotechnology Journal International

Global agriculture is being squeezed from several directions at once: population growth continues, climate variability is accelerating, arable land per capita is shrinking, and micronutrient malnutrition persists alongside outright hunger in many regions. Conventional breeding and first-generation transgenic approaches have delivered real gains over the past century, but both are constrained—breeding by long generation cycles and limited trait precision, transgenics by regulatory friction and uneven public acceptance. Precision genome-editing technologies, spanning zinc-finger nucleases, transcription activator-like effector nucleases, CRISPR-Cas nucleases, base editors, prime editors and RNA-targeting Cas13 systems, have changed what is achievable: breeders and molecular biologists can now introduce defined, heritable genetic changes with a speed and accuracy conventional methods simply cannot match. This review critically synthesises the molecular foundations, agronomic applications, specificity profile, delivery strategies, regulatory trajectories and societal reception of precision genome editing in crop species, with particular attention to disease resistance, abiotic stress tolerance, nutritional biofortification, yield enhancement and the de novo domestication of orphan and wild crop relatives. The empirical evidence on off-target activity, drawn chiefly from whole-genome sequencing studies in rice, cotton and grapevine, indicates that unintended mutations attributable to programmable nucleases are markedly rarer than those introduced by tissue culture and somaclonal variation, although detection methodology and reporting standards remain inconsistent across the literature. Regulatory divergence continues to shape commercial outcomes more than technical feasibility does: several jurisdictions exempt foreign-DNA-free site-directed nuclease products from conventional genetically modified organism oversight, while the European Union has only recently begun moving away from a strictly process-based framework. Consumer survey evidence suggests gene-edited foods are generally, though not universally, viewed more favourably than first-generation genetically modified products, even as public awareness remains low and labelling expectations vary widely across societies. The review concludes that precision genome editing is a necessary, though not on its own sufficient, component of a sustainable intensification agenda for twenty-first-century agriculture, and that its ultimate impact will depend as much on transformation efficiency, regulatory harmonisation and transparent public engagement as on continued molecular innovation.