Green Synthesis of Copper Nanoparticles Using Nyctanthes Arbor-tristis and Study of Its Biomedical Applications
Snehal Kulkarni *
Department of Microbiology, Dr. D. Y. Patil Arts, Commerce and Science College, Sant Tukaram Nagar, Pimpri, Pune, 411018, India.
Neeraj Lokhande
Department of Microbiology, Dr. D. Y. Patil Arts, Commerce and Science College, Sant Tukaram Nagar, Pimpri, Pune, 411018, India.
Akshay Kulkarni
Department of Microbiology, Dr. D. Y. Patil Arts, Commerce and Science College, Sant Tukaram Nagar, Pimpri, Pune, 411018, India.
*Author to whom correspondence should be addressed.
Abstract
Aim: The aim of the present study was to synthesize copper nanoparticles using aqueous extract of leaves of medicinal plant Nyctanthes arbor-tristis using green and ecofriendly method, and to study its bio medicinal application.
Place and Duration of the Study: The study was carried out at Department of Microbiology, Dr. D. Y. Patil ACS College, Pimpri, Pune, Maharashtra State, India during January 2023-May 2023.
Methodology: The present study explores the green synthesis of copper nanoparticle using aqueous extract of the leaves of Nyctanthes arbor-tristis. The biosynthesized copper nanoparticles were characterized using UV-Visible spectroscopy. Further, the antibacterial and antioxidant property of the synthesized nanoparticles was checked which revealed the biomedical application of the nanoparticles.
Results: Green synthesis of copper nanoparticles was done successfully using aqueous extract of Nyctanthes arbor-tristis leaves. The characterization of copper nanoparticles using UV-visible spectroscopy revealed the absorbance peak at 222 nm which confirmed the synthesis of copper nanoparticles. The antibacterial activity against Gram negative bacterium E. coli was found to be significant with 15 mm zone of inhibition and the synthesized copper nanoparticles also showed antioxidant activity through FRAP assay.
Conclusion: Overall, the present research highlighted the green synthesis of copper nanoparticles using medicinal plant Nyctanthes arbor-tristis and it’s characterization using UV-visible spectroscopy The synthesized nanoparticles showed significant antibacterial activity against pathogenic E. coli and antioxidant activity which suggests its biomedical applications.
Keywords: Green synthesis, copper nanoparticles, Nyctanthes arbor-tristis, antibacterial activity, UV-visible spectroscopy