Evaluation of the Antimicrobial Activity of the Two Extracts (Aqueous and Lipidic) of P. aquilinum of Cross Plant
Arnaud W.G. Tamba Sompila *
National Institute for Research in Engineering Sciences, Innovation and Technology, Scientific City, Brazzaville, Congo and National Superior Polytechnic School, Marien Ngouabi University, Brazzaville, Congo.
Nadia. P.G Pambou-Tobi
National Institute for Research in Engineering Sciences, Innovation and Technology, Scientific City, Brazzaville, Congo and National Superior Polytechnic School, Marien Ngouabi University, Brazzaville, Congo.
*Author to whom correspondence should be addressed.
Abstract
Ferns have been living with human beings for a long time. Various biological activities have been reported, such as dietary, antioxidant and others. In our case, the focus was on antimicrobial activity. The aim was to demonstrate the activity of two extracts (aqueous and lipidic) of P. aquilinum fronds. The methodology adopted consisted in extracting the total oil from fiddleheads using a sohlext device, with hexane as the solvent. The aqueous extract was also prepared using water as the extraction solvent. Five strains were selected and used, namely three strains of the Shigella genus: boydii, flexneri and sonnei, and two strains of the Pseudomonas genus: aeruginosa, clinical strain, and aeruginosa, spontaneous flora strain. At a concentration of 250 mg/mL, the lipid extract inhibited the growth of all three Shigella strains, with a mean zone of inhibition of 6.5 mm, 11 mm and 16 mm for Shigella sonnei, Shigella boydii and Shigella flexneri respectively. On the other hand, no activity was revealed against Pseudomonas. On the other hand, for both Shigella and Pseudomonas, the aqueous extract showed no activity at the same concentration of 250 mg/mL.
Keywords: Antibacterial activity, evaluation, extracts, purification, pteridium aquilinum