Optimization of Growth Parameters for Enhancing the Production of Biosurfactants from Pediococcus pentosaceus S-2
Tamanna Kaundal
Department of Biotechnology, Panjab University Research Centre, Goswami Ganesh Dutta Sanatan Dharma College (GGDSD) College, Sector-32, Chandigarh, India.
Anjali Sharma
Department of Biotechnology, DAV College, Sector- 10, Chandigarh, India.
Navneet Batra *
Department of Biotechnology, Panjab University Research Centre, Goswami Ganesh Dutta Sanatan Dharma College (GGDSD) College, Sector-32, Chandigarh, India.
*Author to whom correspondence should be addressed.
Abstract
Numerous Lactic acid bacteria (LAB) have been found to be capable of synthesizing surface-active compounds i.e biosurfactants. These are amphiphilic compounds produced by microorganisms on their cell surface or secreted extracellularly that have a tendency to reduce surface and interfacial tension. In the present study, different process parameters including nitrogen and carbon source, pH, temperature, aeration and agitation were optimized to maximize the production of biosurfactants from Pediococcus pentosaceus S-2. Xylose (1.5%) and yeast extract (1.5%) act as better carbon and nitrogen sources respectively for the production of biosurfactants. Maximum biosurfactant yield was observed at pH 6, a temperature of 35o C, an agitation rate of 200 rpm and with inoculum size of 3%. The high yield of biosurfactants produced from Pediococcus pentosaceus S-2 by utilizing media supplemented with whey under optimized conditions.
Keywords: Biosurfactants, Lactic Acid Bacteria (LAB), optimization conditions, Pediococcus pentosaceus