Isolation of High Lignolytic Bacteria from Termites’s Gut as Potential Booster in for Enhanced Biogas Production
U. S. Anukam *
Department of Microbiology and Biochemistry, Federal Polytechnic, Nekede, Owerri, Nigeria.
J. N. Ogbulie
Department of Microbiology, Federal University of Technology, Owerri, Nigeria.
C. Akujuobi
Department of Microbiology, Federal University of Technology, Owerri, Nigeria.
W. Braide
Department of Microbiology, Federal University of Technology, Owerri, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Bacteria strain capable of degrading lignin, cellulose and hemicellulose were isolated from wood Feeding termite gut using spread plate technique. The 16S rRNA gene sequencing methodology was adopted in the identification of the isolate. The isolate’s Morganella morganii (strain S4L2C (MH745964) were found to have a high lignin degradation potential. The organism was able to reduce the lignin content of rice straw from 17.43% to 7.29% after 30 days of pretreatment with 53.27% reduction of the lignin content. This study revealed that termite’s gut bacteria are Potential sources of lignocellulose degrading bacteria for the biological conversion of biomass to biogas production.
Keywords: Biodelignification, termites gut, gene sequence, biogas