Starch Phosphorylase: An Overview of Biochemical Characterization, Immobilization and Peptide Mapping
Ritu Jain
School of Biotechnology, Devi Ahilya University, Khandwa Road, Indore-452001, India
Sarika Garg
Department of Neuroscience, The University of Montreal Hospital Research Centre (CRCHUM), Bureau/Office: CRCHUM R09.476, Tour Viger, 900, rue St-Denis, Montréal, Québec H2X 0A9, Canada
Anil Kumar *
School of Biotechnology, Devi Ahilya University, Khandwa Road, Indore-452001, India
*Author to whom correspondence should be addressed.
Abstract
Starch Phosphorylase: An Overview of Biochemical Characterization, Immobilization and
Peptide Mapping
Starch phosphorylase (EC 2.4.1.1) is the key enzyme which catalyzes the reversible conversion of starch and inorganic phosphate into glucose-1-phosphate (G-1-P) and has been reported in many higher plants. It can be exploited for industrial purposes for tailoring starch and for the synthesis of biopolymers which may be useful in food industry and are also of clinical importance. Starch in the form of insoluble granules accumulates in chloroplasts as the primary product of photosynthesis. Multiple forms of the enzyme have been reported in different plant tissues which have been predicted to have different roles in starch metabolism. Here, various biochemical properties have been reviewed. Various techniques for enzyme immobilization have been discussed. Besides, reports on immobilization of starch phosphorylase from different sources and on different solid matrices have also been reviewed. Peptide mapping reports of various proteins have also been assessed in this review.
Keywords: Starch phosphorylase, glucose-1-phosphate, biochemical aspects, immobilization, peptide mapping