Rapid micropropagation of Cucumis sativus var. Dastgerdi (Iranian cultivar) by Node Culture Technique
Otroshy Mahmoud *
Department of Tissue Culture, Agricultural Biotechnology Research Institute of Iran (ABRII), P. O. Box 85135-487, Isfahan, Najafabad, Iran
Mokhtari Arash
Department of Tissue Culture, Agricultural Biotechnology Research Institute of Iran (ABRII), P. O. Box 85135-487, Isfahan, Najafabad, Iran
*Author to whom correspondence should be addressed.
Abstract
Rapid micropropagation of Cucumis sativus var. Dastgerdi (Iranian cultivar) by Node Culture Technique
Tissue culture can be used in developing an efficient method for production of valuable and enduring indigenous cultivars. A short-term protocol for plantlet proliferation by using nodal segments was developed for Cucumis sativus, local cultivar from Isfahan; known as Dastgerdi as a tolerant cultivar to the root-knot nematode. Nodal explants were cultured on MS medium containing various concentrations of KIN (0, 0.5, 1 and 1.5mg.l-1) in combination with IBA (0, 0.025 and 0.5mg.l-1). The best response for proliferation rate (100%), mean number of nodes per plantlet (6.01) and percentage of rooted In vitro propagated shoots were observed on medium supplemented by KIN (1.5mg.l-1) after about 3-4 weeks. The maximum length (5.43cm) of micro-shoots obtained on MS medium with KIN (1mg.l-l) + IBA (0.025mg.l-1).
Keywords: Cucumis sativus, IBA, KIN, native cultivar, proliferation