In Vitro Propagation of Alternanthera sessilis L. from Internode Explant
Dimpy Das *
M.C. College, Barpeta, Assam- 781 301, India
P. K. Borua
Department of Life Sciences, Dibrugarh University, Assam- 786 004, India
*Author to whom correspondence should be addressed.
Abstract
An efficient protocol was developed for micropropagation of Alternanthera sessilis L. from internode explant. Callus was developed from the cut ends and surface of the internode explants when the Murashige and Skoog (MS) medium was supplemented with 0.5mg/L or 1.0mg/L 2,4-D. The greatest mean number of shoots (124) with the highest mean shoot length (9.3cm) was obtained when calli were cultured on the MS medium with 1.0mg/L each of 6-Benzylaminopurine and adenine sulfate. Early and maximum root induction was noted when shoots were cultured in half strength MS basal medium. In vitro regenerated plants were successfully hardened in a mixture of soil and cow dung in ratio of 3:1 and transferred to field. Cent percent plants survived in field condition, morphologically identical to parent plant and showed normal flowering.
Keywords: Alternanthera sessilis, medicinal plant, micropropagation, internode, callus, shoot bud induction