Haploid Plant Regeneration from Unpollinated Ovules of Cucumis melo L. Var. Conomon cv. Mudicode
Sunita Premnath Koli
Plant Tissue Culture Laboratory, Department of Botany, Karnatak University, Dharwad – 580003, India
Hosakatte Niranjana Murthy *
Plant Tissue Culture Laboratory, Department of Botany, Karnatak University, Dharwad – 580003, India
*Author to whom correspondence should be addressed.
Abstract
Major objective of the present work was to induce haploid plants in Cucumis melo var. conomon cv. Mudicode (Common name: Kani vellari). Ovules of Kani vellari plants were collected one day before anthesis and were cultured in vitro on MS medium supplemented with IAA, IBA, NAA, 2, 4-D, KN, BAP, TDZ and 2-iP (1.0, 2.0 and 5.0 µM) and BAP/TDZ/KN (1.0, 2.0 and 5.0 µM) in combination with IAA, IBA, NAA, 2, 4-D (1.0, 2.0 and 5.0 µM). The cultured ovules involved in callus induction and an optimum quantity of 93.15 mg of callus from cultured ovules was obtained on the MS medium fortified with 1 µM BAP + 2 µM NAA. The callus induced on medium supplemented with 1 µM BAP+2 µM NAA was subcultured on MS medium supplemented with 5 µM BAP was involved in shoot organogenesis and developed a maximum of 5.55 shoots. The rooting of the regenerated shoots was achieved on MS medium supplemented with 1 µM IAA. Cytological analysis of the root tips of regenerated plants confirmed their haploid nature.
Keywords: Haploids, melon, ovule culture