In vitro and In vivo Effects of the Leaf Extracts of Cassia tora and Cassia sophera in Reducing the Cytotoxicity and Angiogenesis
Sumangala Rao
Department of Biotechnology, Jawaharlal Nehru Technological University, Hyderabad, 500085, India
C. Suresh *
Department of Biochemistry, National Institute of Nutrition, Hyderabad, 500007 Andhra Pradesh, India
*Author to whom correspondence should be addressed.
Abstract
Aim: Consumption of C. tora and C. sophera of family caesalpinaceae as leafy vegetables by local folklore has initiated this study to investigate the cytotoxic effect and antiangiogenic effect of their leaf extracts on human cancer cell lines and chick embryo respectively.
Study Design: For in vitro studies colon and hepatocarcinoma cell lines were used. For in vivo studies chicken egg chorioallantoic membrane (CAM) assay is used.
Methodology: Methanol, ethyl acetate and petroleum ether extracts of leaves were tested for antiproliferative activity using MTT assay, on HCT15 and Hep G2 cell lines. In vivo CAM model was used to study anti-angiogenesis activity of extracts using filter paper discs.
Results: All extracts of both plants were toxic to tested cell lines with increasing concentrations and time. Ethyl extract of Cassia tora was found to be most toxic on Hep G2 cell line, though ethyl extract and methanol extracts of both plants were found to give strong antiproliferative activity. Hep G2 was found to be most sensitive to all extracts than HCT15 as inhibited by low IC50 values. Ethyl acetate extracts of both plants exhibited greater anti-angiogenesis activity than other extracts.
Keywords: C. tora, C. sophera, HCT15, Hep G2, antiproliferative, MTT assay, CAM model, anti-angiogenesis