Preparation of Protein Extraction from Flower Buds of Solanum lycopersicum for Two-Dimensional Gel Electrophoresis
Xiaocui Zhao
Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, China
Jingqi Ren
Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, China
Na Cui *
Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, China and Key Laboratory of Protected Horticulture, Ministry of Education, Shenyang Agricultural University, Shenyang 110866, China
Haiyan Fan
Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, China
Guangchao Yu
Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, China
Tianlai Li
Key Laboratory of Protected Horticulture, Ministry of Education, Shenyang Agricultural University, Shenyang 110866, China
*Author to whom correspondence should be addressed.
Abstract
Aims: Find a suitable method for the protein extraction from flower buds of Solanum lycopersicum.
Study Design: Compare some kinds of protein extraction methods and find the best one among them suitable to tomato flower buds.
Place and Duration of Study: Biological Science and Technology College, between June 2010 and July 2011.
Methodology: The proteins for electrophoresis were extracted using different methods, such as trichloroacetic acid /acetone (TCA/acetone), Sodium dodecyl sulfate (SDS), Tris-saturated phenol (Tris-Phen), Phenol/SDS and Direct lysis method. After silver staining, different patterns of protein spots were observed in the gels.
Results: Few spots were found by SDS and Phenol/SDS extractions, more spots by immediate dissolution but the most impurities, less protein productivity though more spots by Tris-Phen extractions, and more protein productivity and better apart effect by TCA/acetone. The 2-DE image background was the clear and the protein spots were the most by TCA/acetone method.
Conclusion: TCA/acetone method is much more suitable as extraction method for protein two-dimensional electrophoresis of tomato flower buds.
Keywords: Flower buds, protein extraction, Solanum lycopersicum, two-dimensional gel electrophoresis