Cell Proliferation Using Broccoli Leaf Cutting in vitro Culture: Its Biochemical and Antioxidant Properties
A. B. M. Sharif Hossain *
Program of Biotechnology, Department of Biology, Faculty of Science, University of Hail, KSA and Program of Biotechnology, ISB, Faculty of Science, University of Malaya, Kl, Malaysia
Imdadul Haq
Program of Biotechnology, ISB, Faculty of Science, University of Malaya, Kl, Malaysia
Nasir Adam Ibrahim
Program of Biotechnology, Department of Biology, Faculty of Science, University of Hail, KSA
Mohammed Aleissa
Department of Biology, Faculty of Science, AI-imam Muhammad Ibn Saud Islamic University, KSA
*Author to whom correspondence should be addressed.
Abstract
Significance of the study: Tissue or cell culture keeps a significant role in micro-propagation in the plant production industry. Nowadays it has been successfully performed from many species in plant. Millions of explants can be produced by tissue or cell culture per year in any plant production industry.
Aim: The study was conducted to investigate the root, shoot and leaf formation from the leaf cutting in vitro culture of broccoli (Brassica oleracea var italic).
Methodology: Different BAP (6-Benzylaminopurine) and IBA (Indole-butaric-acid) hormone (0.25, 0.50, 1.0, 1.50, 2.0 2.5, 3.0 and 3.5 mg/l) concentrations in combination with MS media was used to culture leaf cutting in vitro.
Results: The results showed that there was no shoot formed, but a few leaves and bulky callus proliferation were appeared. The highest number (6.75) of root proliferation was found in the concentration of 2.0 mg/l IBA + 0.25 mg/l BAP combination. 2,2-diphenyl-2-picrylhydarzyl (DPPH) free radical scavenging potential was higher (70%) in leaves extract than in callus extracts (46%) at concentration of 10 mg/ml.
Conclusion: The present results conclude that it is better to use the combination of BAP and IBA at different concentration to produce root proliferation, leaf initiation and callus formation in broccoli.
Keywords: Cell and tissue culture, leaf tip, IBA, BAP, explants