Exploration of Phytochemical and Antibacterial Potentiality of Anagallis arvensis L. Extract against Methicillin-Resistant Staphylococcus aureus (MRSA)
Javad Sharifi-Rad
Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran and
Seyedeh Mahsan Hoseini-Alfatemi
Pediatric Infections Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abdolhossein Miri
Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran and Zabol University of Medical Sciences, Zabol, Iran
Majid Sharifi-Rad
Department of Range and Watershed Management, Faculty of Natural Resources, University of Zabol, Iran
Marzieh Sharifi-Rad
Department of Chemistry, Faculty of Science, University of Zabol, Zabol 98615-538, Iran
Masoumeh Hoseini
Department of Food Science and Technology, Faculty of Agriculture, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
Mehdi Sharifi-Rad *
Zabol University of Medical Sciences, Zabol, Iran
*Author to whom correspondence should be addressed.
Abstract
Context: Anagallis arvensis L. (Scarlet pimpernel) was used to treatment of several ailments in several countries.
Objective: The aim of this study was to evaluate the in vitro antimicrobial activity of leaf methanolic extract of A. arvensis against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA).
Methods: In this study A. arvensis leaf was shade dried, powdered and extract was made by using methanol. The antimicrobial activity of methanolic extract was investigated against clinical isolates of MRSA by both the disc diffusion method and the microbroth dilution method.
Results: The result of disc diffusion method showed that the plant extract recorded different degrees of antibacterial activity on MRSA as evidenced by the inhibited zones. The MICs of the plant extract and vancomycin were >100 and 14.5±0.1 µg/mL, respectively. This results showed that the plant extract although have slightly effect on MRAS but it was not sufficient strong.
Discussion and Conclusion: A. arvensis leaf extract has anti-MRSA properties, corroborating the traditional therapeutic uses of this plant, and can be used in the therapy of infectious diseases as well as an antimicrobial supplement in food industries.
Keywords: Scarlet pimpernel, Inhibition zone, disc diffusion method, microbroth dilution method, MIC