Gamma Radiation: Physical Elicitor for In vitro Culture Techniques of Cajanus cajan
Darakhshanda Neelam *
Department of Biosciences, University of Jamia Millia Islamia, Jamia Nagar, New Delhi, 110025. India
Tanveera Tabasum
Department of Zoology, College for Women, M.A. Road Srinagar, 190001, India
S. A. Husain
Department of Biosciences, University of Jamia Millia Islamia, Jamia Nagar, New Delhi, 110025. India
Mahmooduzaffar .
Department of Botany, University of Jamia Hamdard, New Delhi, 110062, India
Shahnaz Subhan
Amity Institute of Biotechnology, Amity University, Noida, U.P -201301, India
*Author to whom correspondence should be addressed.
Abstract
Gamma rays are very important in mutation breeding and in in vitro mutagenesis in order to develop required features of plants and increase the genetic variability. Cajanus cajan when subjected to absorbed doses 30 Gy, 50 Gy, 100 Gy, 150 Gy and 200 Gy showed a direct co-relation between callus induction, regeneracy frequency and absorbed doses of gamma radiation as compared to control. Gamma irradiation resulted in the induction of autonomous growth in callus, which led to the formation of callus tumors resembling the shape of crown gall tumors. Gamma irradiation in the present study proved to be an important tool in increasing the breeding efficiency, and regeneration frequency, especially that of the recalcitrant varieties.
Keywords: Callus tumors, regeneracy, crown gall, in-vitro, gamma rays