Effects of AtSPS on the Growth and Development of Arabidopsis thaliana under Abiotic Stress
Bao-Zhen Zhao
College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China.
Yang Yu
College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China.
Zhi Yang
College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China.
Qi Ding
College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China.
Na Cui *
College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China and Key Laboratory of Protected Horticulture of Ministry of Education, Shenyang Agricultural University, Shenyang 110866, China.
*Author to whom correspondence should be addressed.
Abstract
Aims: SPS (Sucrose phosphate synthase) participates in plant growth and yield formation, and plays an important role in plant stress resistance. This study used T-DNA insertion mutant of AtSPS in Arabidopsis as test material. The growth indexes and soluble sugar contents of Arabidopsis thaliana under salt stress, osmotic stress and low temperature stress were determined, which laid the foundation for further understanding the mechanism of SPS in plant growth and development and abiotic stress resistance.
Study Design: In order to analyze the mechanism of SPS in plant growth and development and abiotic stress resistance, this study used T-DNA insertion mutant of AtSPS in Arabidopsis as test material. The growth indexes and soluble sugar contents of Arabidopsis thaliana under salt stress, osmotic stress and low temperature stress were determined.
Place and Duration of Study: College of Biological Science and Technology, between December 2020 and May 2021.
Methodology: The contents of soluble sugar in tomato fruits were measured with HPLC (High
performance liquid chromatography). The growth indexes were determined.
Results: The results showed that AtSPS played positive regulation roles in seed germination and seedling growth of Arabidopsis thaliana. However, under abiotic stress conditions, AtSPS mutant increased the contents of soluble sugar, suggesting that Arabidopsis thaliana seedlings might improve resistance through osmotic regulating substances.
Conclusion: AtSPS played positive regulation roles in seed germination and seedling growth of Arabidopsis. Meanwhile, AtSPS mutant increased the contents of soluble sugar to increase resistance of Arabidopsis under abiotic stresses, and the growth and development were blocked, suggesting that SPS was negative regulatory element to resist abiotic stress.
Keywords: Arabidopsis thaliana, sucrose phosphate synthase, AtSPS, ABIOTIC stress