Effect of BAP and IBA on in vitro Regeneration of Local Banana Variety of Sabri
Fahima Khatun *
Department of Biotechnology, Sher-e-Bangla Agricultural University, Bangladesh
M. E. Hoque
Department of Biotechnology, Sher-e-Bangla Agricultural University, Bangladesh
Homayra Huq
Department of Biotechnology, Sher-e-Bangla Agricultural University, Bangladesh
Md. Adil
Department of Plant Breeding, Bangladesh Rice Research Institute, Bangladesh
Kh. Ashraf-Uz-Zaman
Department of Agricultural Chemistry, Sher-e-Bangla Agricultural University, Bangladesh
Mominul Haque Rabin
Department of Agricultural Chemistry, Sher-e-Bangla Agricultural University, Bangladesh
*Author to whom correspondence should be addressed.
Abstract
The experiment was carried out to study the effect of benzylaminopurine (BAP) (1.0, 2.0, 3.0, 4.0 and 5.0 mg/L) and indole-3-butyric acid (IBA) (0.5, 1.0, 1.5, 2.0 and 2.5 mg/L) for in vitro regeneration of sabri variety of Banana using shoot tip explants. Highest response of explants (84%) and maximum number of shoots per explant (3.4) were observed with 5.0 mg/L BAP in sabri. In contrast, due to combined effect, 5.0 mg/L BAP+2.5 mg/L IBA showed best response (90%). The highest shoot number per explant (3.4) was found with 5.0 mg/L BAP+2.0 mg/L IBA. The maximum number of roots (3.4 and 5.2) was observed in 1.5 mg/L and 3.0 mg/L BAP+1.0 mg/L of IBA. In controlled environment, the regenerated healthy rooted plantlets were transferred from culture media to soil in plastic pots where 90% plantlets were survived and in open atmosphere, the survival rate was 88.89%. This protocol has the applicability in vitro rapid propagation of Banana.
Keywords: In vitro, regeneration, BAP, IBA, survival rate