Isolation and Identification of Endophyte Microorganisms from Bauhinia monandra Leaves, Mainly Actinobacteria
Rosilma de O. Araujo-Melo *
Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco (UFPE), Avenida dos Economistas, S/N, Cidade Universitária, Recife-PE, CEP 52171-011, Brazil
Igor F. A. C. Souza
Faculdade Integrada de Pernambuco, Avenida Caxangá, nº 4477, Várzea, Recife-PE, CEP 50740-000, Brazil
Carlos Vinícius J. de Oliveira
Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco (UFPE), Avenida dos Economistas, S/N, Cidade Universitária, Recife-PE, CEP 52171-011, Brazil
Janete M. de Araújo
Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco (UFPE), Avenida dos Economistas, S/N, Cidade Universitária, Recife-PE, CEP 52171-011, Brazil
Kêsia X. F. R. de Sena
Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco (UFPE), Avenida dos Economistas, S/N, Cidade Universitária, Recife-PE, CEP 52171-011, Brazil
Luana Cassandra Breitenbach Barroso Coel
Departamento de Bioquímica, Centro de Biociências, UFPE, Avenida Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brazil
*Author to whom correspondence should be addressed.
Abstract
The present work aimed the prospection of microorganisms from Bauhinia monandra leaves, with the purpose to identify endophytics to obtain strains with possible biotechnological applications. B. monandra leaves, disinfected with hypochlorite solution, were macerated in phosphate buffered saline and seeded in ten culture media containing antibacterial or antifungal agents. The endophytic filamentous fungus strains detected belonged to the genera Penicillium, Curvullaria and Aspergillus. Non-filamentous endophyte bacteria were grouped in the genera Bacillus, Burkholderia, Enterobacter and strains of endophytic Actinobacteria were classified as Streptomyces and Nocardiopsis. The isolation of endophytic microorganisms with nine culture media revealed better bacteria development with L-arginine agar; inorganic salt starch agar and potato dextrose agar were superior to Actinobacteria and fungus strains, respectively. The present study revealed the predominance of the genus Penicillium in leaves of B. monandra. This work introduces the first data of identification from endophyte Actinobacteria in the leaves of B. monandra.
Keywords: Endophytes, microorganisms, Bauhinia monandra, leaves, actinobacteria