In-vitro Measurement of Glucose Concentration in Human Blood Plasma Mixed Intralipid Phantom Samples by Using Modulated Ultrasound and Infrared Light
Anuj Srivastava *
School of Biomedical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi, Uttar Pradesh-221005, India
Md. Koushik Chowdhury
School of Biomedical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi, Uttar Pradesh-221005, India
Shiru Sharma Sharma
School of Biomedical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi, Uttar Pradesh-221005, India
Neeraj Sharma
School of Biomedical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi, Uttar Pradesh-221005, India
*Author to whom correspondence should be addressed.
Abstract
Non-invasive blood glucose measurement is one of most innovative domain in Biomedical Engineering. Multiple methodologies have-been introduced over last few decades to fulfil the clinical requirement for non-invasive glucose measurement in human beings, however, without a successful breakthrough. This research article uses modulated ultrasound with infrared light-based technique to study glucose-induced variations in human blood plasma mixed IntralipidTM phantom samples using infrared light of 940 nm and 40 kHz central frequency based ultrasonic transmitter unit. The test uses blood samples of 30 study subjects during oral glucose tolerance test and fasting, postprandial and random stages based blood glucose tests respectively. The result as obtained from oral glucose tolerance tests and fasting, postprandial and random stages blood glucose tests showed peak amplitude values in Fast Fourier Transform domain varies in corresponding to blood glucose levels in in-vitro samples. The Bland Altman plot, Error Grid and statistical analysis represent the potentiality and feasibility of our technique for non-invasive blood glucose measurement.
Keywords: Non-invasive, modulated ultrasound, infrared light, IntralipidTM phantom, blood glucose