Molecular Biology of Microbial Pectate Lyase: A Review
Amit K. Dubey
Department of Biotechnology, D. D. U Gorakhpur University, Gorakhpur (U.P.) 273 009, India
Sangeeta Yadav
Department of Biotechnology, D. D. U Gorakhpur University, Gorakhpur (U.P.) 273 009, India
Manish Kumar
Department of Biotechnology, D. D. U Gorakhpur University, Gorakhpur (U.P.) 273 009, India
Gautam Anand
Department of Biotechnology, D. D. U Gorakhpur University, Gorakhpur (U.P.) 273 009, India
Dinesh Yadav *
Department of Biotechnology, D. D. U Gorakhpur University, Gorakhpur (U.P.) 273 009, India
*Author to whom correspondence should be addressed.
Abstract
Pectate lyase represents an important member of pectinase group of enzymes responsible for the pathogenesis and softening of plant tissues. It also has role in fruit juice clarification and in retting of natural fibers. The biochemical characterization of pectate lyases from diverse microbial sources and plants along with an insight to the protein structure has been dealt earlier but there is a lack of exclusive review on the molecular biology of pectate lyases. This review tries to fill the gap by highlighting the various aspects of molecular biology of microbial pectate lyases especially the cloning and expression of pectate lyase genes from diverse sources attempted so far. The topics covered in this review are a brief description about enzymes associated with degradation of pectin, its classification, applications, updated information about the biochemical characterization of microbial pectate lyases and cloning and expression of microbial pectate lyase genes.
Keywords: Pectin, pectinases, pectate lyases, molecular biology, cloning, genes