Fruit Peel from Astrocaryum aculeatum G. Mey. AS Substrate for Lipases Production by Aspergillus niger A2B1
Nayara Fátima Lazameth Diniz
Laboratório de Micologia, Instituto Nacional de Pesquisas da Amazônia (INPA), Brazil.
Rossicléa Gonzaga da Silva
Laboratório de Micologia, Instituto Nacional de Pesquisas da Amazônia (INPA), Brazil.
Luciana Aires Oliveira
Laboratório de Micologia, Instituto Nacional de Pesquisas da Amazônia (INPA), Brazil.
Waldireny Rocha Caldas
Instituto de Saúde e BIotecnologia-ISB, Universidade Federal do Amazonas, Brazil.
Érica Simplício de Souza
Escola Superior de Tecnologia, Universidade do Estado do Amazonas, Brazil.
Roberto Figlioulo
Coordenação de Tecnologia e Inovação Instituto Nacional de Pesquisas da Amazônia (INPA), Brazil.
Juliana Gomes de Souza Oliveira
Laboratório de Micologia, Instituto Nacional de Pesquisas da Amazônia (INPA), Brazil.
João Vicente Braga de Souza *
*Author to whom correspondence should be addressed.
Abstract
The fruit of Astrocaryum aculeatum G. Mey. are consumed by population in the Brazil Northern resulting fruit peels. These peels are rich in lignocellulose and fat. The present study investigated peels from the Astrocaryum aculeatum G. Mey. as a substrate for lipases production by solid state bioprocess. To reach this objective: 1) we isolated fungi from peels from the fruit of Astrocaryum aculeatum G. Mey; 2) we screened the isolates for lipase production (screening in petri dish and screening in submerged bioprocess), 3) we investigated the production of lipases by using peels from Astrocaryum aculeatum G. Mey as substrate. The isolates belonged to the genera Aspergillus (16), Penicillium (3) and Fusarium (1). These strains were submitted to petri dishes and submerged fermentation for lipases production, these experiments resulted in the selection of five strains belonging to the Aspergillus genera. The lipases produced by these five strains performed enzymatic transesterification; however, the lipases from the strain Aspergillus niger A2B1 produced the highest ester content. The utilisation of fruit peel from Astrocaryum aculeatum G. Mey. as the main substrate, fruit peel from Astrocaryum aculeatum G. Mey. oil (13%), moisture (70%) and 75 h of incubation were the optimal conditions identified for the production of lipases by Aspergillus niger A2B1(17.42 U/g) in solid state bioprocess (SSB).
Keywords: Tucumã peel, bioprocess, lipases, enzymatic transesterification.