Clonal Propagation of Strawberry (Fragaria x ananassa Duch.) through In vitro Runner Tip Culture through Incorporation of Growth Hormones
Md. Nasir Uddin Badal *
Department of Genetic Engineering and Biotechnology, Jessore University of Science and Technology, Jessore 7408, Bangladesh and Protein Dynamics Research Group, Faculty of Medicine and Life Science, University of Tampere, Tampere 33520, Finland.
Md. Shoyeb
Department of Genetic Engineering and Biotechnology, Jessore University of Science and Technology, Jessore 7408, Bangladesh.
Md. Abdur Rauf Sarkar
Department of Genetic Engineering and Biotechnology, Jessore University of Science and Technology, Jessore 7408, Bangladesh and Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University, Saga 840-8502, Japan.
Md. Shahedur Rahman
Department of Genetic Engineering and Biotechnology, Jessore University of Science and Technology, Jessore 7408, Bangladesh.
Shaikh Mizanur Rahman
Department of Genetic Engineering and Biotechnology, Jessore University of Science and Technology, Jessore 7408, Bangladesh.
*Author to whom correspondence should be addressed.
Abstract
Runner tips explants of strawberry give rise to multiple shoots when cultured on MS medium supplemented with different concentrations and combinations of BAP with KIN or NAA or GA3.The highest response of shoot multiplication was obtained on MS containing 2.5 mgl-1 BAP + 0.5 mgl-1 Kin + 0.5 mgl-1 GA3. The maximum frequency of rooting (83%) and highest number of roots (3.49) was produced in medium containing 1.0 mgl-1 IBA. The well grown rooted plantlets were acclimatized and successfully established in autoclaved vermiculate soil and as well as natural condition. Using our established protocol, it is also possible to provide large numbers of micropropagated plantlets of this cultivars to produce high quality strawberry fruit for commercial cultivation practices.
Keywords: Strawberry, In vitro, regeneration, acclimatization multiple shoots and roots.